AB0300 REDUCTION OF LACHNOSPIRA IS CLOSELY RELATED TO AUTOIMMUNE DISEASE COMPLICATED WITH PULMONARY FIBROSIS

Background Autoimmune diseases (AD) are a group of heterogeneous disorders caused by both genetic and environmental factors. Rheumatoid arthritis (RA) Sjögrens syndrome (SS) typical autoimmune diseases[1], Pulmonary fibrosis (PF) is the most common complication AD. Despite extensive study human gut microbiome in AD complicated with PF(AD-PF),the question whether there microbial features characterizing AD-PF still remains[2]. Objectives This focused on exploring differences between microbiota diversity peripheral lymphocyte subpopulations as well cytokine PF different from that without PF. Methods A total 64 patients (44 20 PF) 100 age- sex- matched healthy controls (HCs) were enrolled this study. The subsets analyzed flow cytometry investigated via 16s rRNA sequencing. Alpha Beta (bray curtis distance-based) analysis was used to define difference profiles HCs. To explore specific bacterial taxa associated AD-PF, STAMP software compare fecal composition. Spearman correlation determine similarities community clinical meatures among samples. Results There decrease richness index HCs, patients. Principal co-ordinates analyses suggested these three states explained reasonable proportion observed variance composition (ANOSIM R2 = 0.113, p < 0.001; Figure 1b). Compared obvious 19 species flora at genus level, which 2 (Lachnospira,Muribaculaceae) belong showed much fewer. relative abundance Lachnospira positive correlated absolute numbers Th17, IL-6 THF-α (P<0.05,Figure 1D-E)，which indicated may be critical AD-RF patients’ own flora. Therefore, reduction influence immune status intestinal tract producing less short-chain fatty acids. Conclusion Our results suggest lead occurrence pulmonary interstitial fibrosis, closely Cytokines, maintaining balance might potential therapeutic target for AD-PF. References [1]Ma Y, Shi N, Li M, Chen F, Niu H: Applications Next-generation Sequencing Systemic Diseases . Genomics Proteomics Bioinformatics 2015, 13 (4):242-249. [2]Belkaid Hand TW: Role immunity inflammation Cell 2014, 157 (1):121-141. Acknowledgements work supported National Natural Science Foundation China (No. 82001740). Disclosure Interests None declared